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The ELISA Guidebook: Second Edition (Methods in Molecular Biology) Paperback – 28 Oct 2010


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Product details

  • Paperback: 566 pages
  • Publisher: Humana Press; Softcover reprint of hardcover 2nd ed. 2009 edition (28 Oct. 2010)
  • Language: English
  • ISBN-10: 1617378844
  • ISBN-13: 978-1617378843
  • Product Dimensions: 19.3 x 3.4 x 26 cm
  • Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
  • Amazon Bestsellers Rank: 1,991,503 in Books (See Top 100 in Books)
  • See Complete Table of Contents

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Review

From the reviews of the second edition:

"The book contains detailed instructions and extensive illustrations with many practical examples on the technique permitting even investigators not familiar with the technique to learn all the basic concepts and practical experimental details … and apply ELISA methodology successfully in basic and clinical research. … The book is written mainly for molecular and cellular biologists, biochemists, pharmaceutical scientists, immunologists, pathologists, laboratory medicine researchers, and all researchers using biochemical assays. … This volume … is an excellent manual for the understanding and development of the ELISA." (Ruben Mestril, Doody’s Review Service, May, 2009)

"It is the practical ELISA bible, a definitive manual covering theoretical aspects, commercial reagents, troubleshooting, instrumentation, enzyme-linked conjugates and microwell plates. … Throughout this book excellent use is made of flow diagrams, graphs and tables to aid the reader. … The ELISA Guidebook is a must for institutional libraries, departments of immunology, hospital diagnostic laboratories, research laboratories using ELISA-based assays and companies developing ELISA immunodiagnostic kits. The book is well worth its price." (Cyril J. Smyth, Microbiology Today, November, 2009)

“The 2nd Edition of The ELISA Guidebook is essentially the author’s tribute to the ELISA technology that has revolutionised all laboratories … . This edition well lives up to its name … . Overall, I rate this book as an extremely valuable resource … to the daily ELISA user. I recommend this book to all Biomedical Scientists, Clinical Scientists and trainees in Laboratory Medicine and that one copy should be available in every laboratory that makes extensive use of ELISA.” (Sujoy Khan, Immunology News, November, 2009) “The book describes the methods involved in heterogeneous enzyme-linked immunosorbent assays (ELISA) that provide ideal systems for dealing with a wide range of studies in many biological areas. … This method is widely using in various scientific and industrial areas … . provides a premier practical guide to the understanding and application of ELISA. Each method is described in great detail, and the whole book is an excellent manual for many researchers in biochemistry, molecular biology, immunochemistry, biotechnology, and medicine.” (G. Ya. Wiederschain, Biochemistry, Vol. 9, 2009)

From the Back Cover

The Enzyme Linked Immunosorbent Assay (ELISA) continues, as predicted, to be the predominant assay used in biomedical and agricultural research. While little has changed in the principles of the ELISAs used since the publication of The ELISA Guidebook, many technological advantages, including the increasing number of reagents available for exploitation and the larger spectrum of instruments to measure the coloured products of ELISAs, have greatly benefited the field. Other testing technologies using molecular techniques, predominantly the conventional and more and more often, real time PCR, are rapidly evolving to help diagnose and differentiate disease agents and identify substances present in exquisitely small amounts. The ELISA is still needed either alone in mass screening, such as serological assays, or used in tandem with molecular techniques to fully evaluate the molecular and antigenic nature of analytes.

John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. This Edition enlarges on charting methods for assessing the indirect ELISA, ruggedness and robustness of tests, aspects of kit use and validation, and internal quality control and external quality management of data. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams.

With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook, Second Edition offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply ELISA methodology successfully in day-to-day basic and clinical research.


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Most Helpful Customer Reviews

By Dr Stephen Berezenko on 11 Nov. 2014
Format: Paperback Verified Purchase
An excellent handbook from the absolute basics, through the simplest calculations, useful tips, troubleshooting, validation and data trending.
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Most Helpful Customer Reviews on Amazon.com (beta)

Amazon.com: 3 reviews
9 of 9 people found the following review helpful
Good but annoying 25 Jun. 2008
By Erwin H. Abel - Published on Amazon.com
Format: Paperback
I'm not one for writing reviews but I just had to write this. This book does have useful info but it is horribly written and badly edited. Definitions and explanations on topics are given pages after their introduced. Tables and figures are horribly labeled, if labeled at all. Example I'm currently on pg 226 and table 17 is completely wrong, read the description of the experiment. Another example pages 221 and 222 have identical figures but only one is labeled correctly, page 218 an equation is given which is completely wrong, try it. There are even missing figures. This has got to be one of the most annoying and frustrating texts I have ever read.
3 of 3 people found the following review helpful
edited, really? 20 July 2010
By C Pical - Published on Amazon.com
Format: Hardcover
Agree with the previous reviewer. I cannot believe this book, a second edition at that, has been read and edited by anyone. Glad I did not buy the book. Will see if I get any useful information out of it or lose patience.
One sentence I just read (page 84): "The rows can be assessed for a maximum color in which there is a range of values that are similar". What does this mean????
Four Stars 17 Jan. 2015
By ella solway - Published on Amazon.com
Format: Paperback Verified Purchase
Good reference book.
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