- Paperback: 533 pages
- Publisher: Academic Press Inc; 2nd Revised edition edition (1 Oct 1998)
- Language English
- ISBN-10: 0122496957
- ISBN-13: 978-0122496950
- Product Dimensions: 22.8 x 16.8 x 2.4 cm
- Average Customer Review: 5.0 out of 5 stars See all reviews (1 customer review)
- Amazon Bestsellers Rank: 4,131,811 in Books (See Top 100 in Books)
- See Complete Table of Contents
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Product Description
Review
Each of the protocols is written relatively independent of the rest of the text, allowing the reader to immediately begin using a procedure without having to study the text from cover to cover... Very few other molecular biology guide books are available that cover the isolation and manipulation of RNA as exhaustingly as does this one. This book would be highly valuable in any research facility engaged in molecular biology. --Mark A. Jandreski, Loyola University Medical Center, DOODY'S ELECTRONIC JOURNAL
Product Description
This laboratory guide is a collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. This edition includes a variety of RNA-based PCR techniques, such as differential display PCR, and transcript quantification by competitive (mimic) PCR. The book focuses on how these methodologies can be used in the study of transcriptional and posttranscriptional regulation of eukaryotic genes. The liberal incorporation of flow charts, tables, and graphs facilitate learning and assist in the planning phases of projects. It is a valuable resource for investigators who wish to characterize cellular biochemistry.
Inside This Book
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First Sentence
All cell and tissue functions are ultimately governed by gene expression; therefore, the reasons for electing to study the modulation of RNA as one parameter of cellular biochemistry may be as diverse as the intra-cellular RNA population itself. Read the first page
Front Cover | Copyright | Table of Contents | Excerpt | Index | Back Cover
All cell and tissue functions are ultimately governed by gene expression; therefore, the reasons for electing to study the modulation of RNA as one parameter of cellular biochemistry may be as diverse as the intra-cellular RNA population itself. Read the first page
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Front Cover | Copyright | Table of Contents | Excerpt | Index | Back Cover
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Customer Reviews
Most Helpful Customer Reviews
1 of 1 people found the following review helpful
Format:Paperback
If you need to work with RNA this book has it all. It includes detailed information on Northerns, RT-PCR, RNA protections to name a few. Most techniques get a chapter and there is lots of sumplimental information on the use of stains, RNase contamination even how to make DEPC water. However the best thing about this book is the ease and simplicity with which everthing is described. Nevertheless the easy style covers a wide range of topics in great clarity and deapth.
A Laboratory must have!
Most Helpful Customer Reviews on Amazon.com (beta)
Amazon.com:
2 reviews
5 of 5 people found the following review helpful
great methods book
15 May 2000
Format:Paperback
This book is exactly what it claims to be--a laboratory guide. It provides methods that are clear, but also explains the reasons for the methods. For example: when is an RNA probe better than a DNA probe and why; when nonisotopic labels can be used effectively. Although there is not a chapter on in situ hybridization, I found the section on probes very helpful. There is also a handy glossary at the end and a primer on RNA at the beginning of the book. Dr Farrell realizes the reader maybe new to the field and so doesn't leave out the basics. However, I believe this book will be helpful to all but the most knowledgable molecular biolgists.
1 of 2 people found the following review helpful
Not advanced and back-dated
2 Dec 2004
Format:Plastic Comb
I am a RNA-chemist and I bought this book from amazon.com to get help for my day-to-day research. The moment I had my first glance at this book I was frustrated. This book has described fairly old methods but no contemporary techniques. This book is not even descrided the techniques in an advanced manner which is expected for a book based on so specific field. I would not recommend this book to anyone who has atleast the basic working knowledge of molecular biology.
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