I'm on my first week of class and this is a bad sign of things to come. I am a studious student - an avid textbook reader. Learning has generally been a meditative, pleasurable act for me. Not so with this book! I should have taken a hint when the instructor told us that the most requested tool from their previous students was a reading guide. It's not that these college upperclassmen can't read, this book is really THAT poorly written. Let me demonstrate:
"Another way of characterizing and tracking a segment of DNA is to use a restriction fragment length polymorphism (RFLP). In Chapter 1, we learned that restriction enzymes are bacterial enzymes that cut DNA at specific base sequences in the genome (really? I don't remember reading about restriction enzymes and I've read chapter 1 twice. Why not just tell me what it is rather than make me feel like an idiot?). The target sequences have no biological significance in organisms other than bacteria; they are present purely by chance (I am not sure what the author is pondering here). Although the target sites are generally found consistently at specific locations, sometimes, on any one chromosome, a specific target site is missing or there is an extra site (wait wait, what happened to restriction fragment length polymorphism? are you going to explain that?). If such a site flanks the sequence hybridized by a probe, then a Southern hybridization (did not talk about "southern hybridization" before, only "southern blot") will reveal an RFLP (what site? the site that could be cut out by restriction enzyme?). Consider this simple (this word makes me feel dumb) example in which one chromosome of one parent contains an extra site not found in the other chromosomes of that type in that cross: [inserting confusing looking diagram here]"
If you understood that, good for you. For me, it's like trying to pitch a tent on a small cliff amidst a thunderstorm while warding off fire ants. I don't recommend it.