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Introducing Proteomics: From Concepts to Sample Separation, Mass Spectrometry and Data Analysis Paperback – 4 Feb 2011

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Product details

  • Paperback: 296 pages
  • Publisher: Wiley-Blackwell; 1 edition (4 Feb 2011)
  • Language: English
  • ISBN-10: 0470035242
  • ISBN-13: 978-0470035245
  • Product Dimensions: 19 x 1.6 x 24.6 cm
  • Average Customer Review: 5.0 out of 5 stars  See all reviews (1 customer review)
  • Amazon Bestsellers Rank: 636,497 in Books (See Top 100 in Books)
  • See Complete Table of Contents

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Product Description


"He introduces undergraduate students to the general principles and methods of the field without delving very deeply into any of the details. Graduate students and researchers could also use the book to refresh their memory or catch up with recent developments." (Booknews, 1 June 2011) --This text refers to the Hardcover edition.

From the Back Cover

Introducing Proteomics provides an accessible introduction to current proteomics technology, including all of the background information necessary to enable readers to plan, prepare and analyze proteomics studies. Engaging and packed with expert advice, this indispensable guide will appeal to both students and researchers new to the field alike.

Key Features:

  • Contemporary, broad coverage covers every important aspect of this rapidly changing field from 2–d gel electrophoretic methods, peptide based shotgun proteomics, the main ionization methods and detailed mass spectrometric principles and applications as well as an introduction to the use of protein/RNA and DNA databases to data analysis and the main features of proteomics data mining software.
  • Coherent and student friendly focuses on what readers need to know about each area of proteomics whilst providing pointers towards more specialized literature for those that are keen to find out more.
  • Relevant explores the power and limitations of current proteomics approaches throughout the book to give readers a realistic idea about which results and challenges to expect from any combination of technologies. The concluding part of the book explores real–world case studies to illustrate how proteomics technologies perform in practice whilst dedicated chapters focus on the analysis of post translational modifications and biomarker discovery.
  • Companion website provides a range of problems for self testing with answers, extensive references and links to additional, key proteomics resources.

I think this book will be a popular and valuable resource for students and newcomers to the field who would like to have an overview and initial understanding of what proteomics is about. The contents are well organized and address the major issues. Professor Walter Kolch, Director, Systems Biology Ireland & Conway Institute, University College Dublin

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Most Helpful Customer Reviews

Format: Paperback
The definitive book on proteomics. No other text book covers the contents inside. Clearly written throughout with excellent figures to help inform the reader. A must to help explain complex topics such as mass spec etc.
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Most Helpful Customer Reviews on (beta) 2 reviews
3 of 3 people found the following review helpful
Great book!! 18 Jun 2013
By H. Medford - Published on
Format: Paperback Verified Purchase
Came recommended by one of the leaders in LCD-MS/MS; she has all of her lab members read it. Plenty of detail for beginners like me, but I imagine this would also be an excellent reference guide for those with more MS experience.
3 of 3 people found the following review helpful
Thorough introduction for scientists 1 Sep 2011
By Sarah - Published on
Format: Paperback Verified Purchase
I got this book because I didn't know very much about proteomics aside from "do some 2D gels and some mass spec." This book is primarily an overview of modern technology, techniques, and databases, which are discussed in chapters 1-4. The last chapter is a snapshot of some recent publications in proteomics, as well as future directions of the field.

Things I appreciated in this book include: no background on proteins (translation or modification); overview of strengths and weaknesses of various systems and methods; clear definitions of MALDI, ESI, MS/MS, and ion traps; extensive list of protein databases, with a summary/review for each.

Things I did not appreciate include: somewhat awkward organization (I would recommend chapter 1, 4, 3/2, then 5, otherwise some information in the earlier chapters might be confusing); unhelpful or busy diagrams; the beast of a chapter that is chapter 3 (overview of mass spec technology). Chapter 3 was extremely helpful, but it was also a bit of a drudge.

I still don't feel entirely confident that I would be able to analyze any mass spec data, but I have a much better idea of how it is obtained and what to look for. So overall, very helpful. I would recommend this to other people (with a basic understanding of science) who are interested in the nuts and bolts of proteomics.
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