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Gene Transfer to Plants (Springer Lab Manuals) [Paperback]

Ingo Potrykus , German Spangenberg

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Book Description

4 Oct 2013 3642489672 978-3642489679 1995
Gene Transfer to Plants Within a decade of the first recoveries of transgenic "model" plants, gene trans­ fer is an established and routine technique in numerous laboratories around the world. It contributes to the rapid progress in basic and applied plant sciences in disciplines as diverse as biochemistry, physiology, developmental biology, breeding, food sciences, and biotechnology (Lindsey 1992; Willmitzer and Topfer 1992; Kishore and Somerville 1993; Nessler 1994). Following years of unsuccessful experiments with variations in feeding iso­ lated DNA to plant tissues and organs, gene transfer became a reality soon after it was discovered that the soil bacterium Agrobacterium tumefaciens contained a plasmid, part of it being transferred to competent plant cells (see Hooykaas; Introduction I: Agrobacterium tumefaciens, a natural vector system). Host range limitations of Agrobacterium-mediated gene transfer prompted the search for alternative gene transfer systems, leading soon to the development of "direct gene transfer to protoplasts" (see Potrykus; Introduction III: Direct gene trans­ fer to protoplasts). Further limitations in both gene transfer systems led to the exploration of a great variety of further approaches such as pollen transforma­ tion, pollen tube pathway, electrophoresis, microlaser, liposome-fusion and -injection, macroinjection, direct DNA application in numerous variations, etc. None of these approaches has, so far, been developed to a reproducible gene transfer technique and it is questionable whether they ever led to transformation (for a detailed assessment see Potrykus 1990).

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"The protocols are described as step-by-step procedures in such a way that everybody with some basic knowledge in biological experimentation will be able to repeat the experiments without much further reading."
Journal of Plant Physiology
"...well worth the investment."
Plant Growth Regulation

From the Back Cover

Using the clearly structured protocols given in this manual, it will be easy to apply the latest techniques in plant biotechnology in order to create new plant varieties or varieties with altered and optimized characteristics.
Direct gene transfer into plant cells or protoplasts by the use of microinjection, electroporation or biolistic systems, or mediated by Agrobacteria is described in detail for various plant species, including relevant crops and cereals. Also included are protocols of the standard molecular techniques for the analysis of transgenic plants as well as a section on biosafety considerations and regulations.

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