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Enzyme Kinetics and Mechanism [Hardcover]

Paul F. Cook , W. W. Cleland

Price: 53.00 & FREE Delivery in the UK. Details
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Book Description

22 Mar 2007 0815341407 978-0815341406

Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics.  Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. 

Practical examples taken from the literature demonstrate theory throughout.  The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. 

Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level.  Enzymologists and molecular biologists will find the text a useful reference.

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"Enzyme Kinetics and Mechanism is an advanced text with meticulous details...[that] gives 'hands on' explanations to the target readership..."

Journal of the Higher Education Academy Physical Sciences Centre, June 2008

About the Author

Paul F. Cook is the Grace B. Kerr Centennial Professor of Chemistry and Biochemistry at the University of Oklahoma. His awards and achievements twice included the Alexander von Humboldt Research Fellowship and he was the recipient of the National Institutes of Health Research Career Development Award from 1983 to 1988. Professor Cook is Associate Editor of Protein and Peptide Letters and Executive Editor of Proteins and Proteomics.

W.W. Cleland is the M.J. Johnson Professor and Steenbock Professor of Chemical Science at the Enzyme Institute at University of Wisconsin-Madison. In the early 1960s, Cleland's landmark papers created a theoretical framework for the study of steady-state kinetics of multisubstrate enzymes and laid the foundation for all subsequent steady-state studies. He serves on the editorial boards of Journal of Biological Chemistry and of Biochemistry. Recognized by many as the "father of modern enzyme kinetics," Professor Cleland was elected to the National Academy of Sciences in 1985.


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Amazon.com: 4.0 out of 5 stars  4 reviews
16 of 17 people found the following review helpful
4.0 out of 5 stars Mainly steady-state, and entirely chemical 10 Jan 2008
By John Duncan - Published on Amazon.com
Format:Hardcover|Verified Purchase
The senior author (Cleland) had perhaps the greatest influence of anyone on the practice of enzyme kinetics during the 1960s and 1970s, as witnessed by the very large number of papers from that period that acknowledge his contribution to the work presented, and by the large number of papers that continue to use his terminology and way of representing reaction mechanisms. (Younger biochemists will perhaps not know that he gave the ping pong mechanism its name.) However, until now his recommendations on the subject were not conveniently gathered together in one book, and it was necessary either to go back to his well known series of papers published in 1963 or to various later review articles. This book will be welcome, therefore, to anyone in search of a modern source.

It is a measure of how little the subject has changed that a large part of the book could have been written in 1963; indeed, it gives the impression that large parts of it were indeed written then. The major advance that is covered in detail is the development of the use of isotopes for studying enzyme mechanisms, including both the use of isotopes as labels and the use of kinetic isotope effects as mechanistic probes. Around 100 pages, or about a quarter of the book, are devoted to these topics, and for readers already thoroughly conversant with the 1963 papers these pages will constitute the most valuable part, the part they are most likely to consult in the future. As both authors have made important contributions to the use of isotopes, it is no surprise that the treatment is masterly.

Some other parts of the book are more disappointing. The treatment of pre-steady-state kinetics is superficial: the major methods, such as stopped-flow, rapid quenching and relaxation (temperature jump etc.) are briefly described, but with little indication of what they are useful for. Similar criticisms can be, and indeed often are, made about most books on enzyme kinetics that have a primary emphasis on the steady state, but one could have hoped for more.

In the entire book there is just one sentence (in the Preface, on page xvii) that mentions that enzymes are of interest not just to chemists but that they also have physiological roles to fulfil. Not surprisingly, therefore, although there are a few pages that mention the existence of cooperative and allosteric effects, there is no indication of what functions these might have in the living organism, and no discussion of the kinetics of multi-enzyme systems.
1 of 1 people found the following review helpful
2.0 out of 5 stars not as much as hoped 18 Oct 2013
By sdrain - Published on Amazon.com
I want to improve my enzyme kinetics, so have been looking for books that are helpful. Found this one at amazon. Because the price tag is not small, I decided to rent it from library to have a feeling first. Tons of equations without explanation how they are derived. Although references are provided, it really diminishes the value of being a book: not need to look elsewhere much.
I believe if a couple of equations can be derived in details as examples. It will make a big difference in people's understanding and will be easy for readers to follow when it goes to later and later, such as the isotope labeling.
I never bought the book.
1 of 2 people found the following review helpful
5.0 out of 5 stars My Bible 6 Sep 2011
By Vidya P. Kumar - Published on Amazon.com
Format:Hardcover|Verified Purchase
This book is awesome. It is hard to read in the beginning but eventually I realized there is so much information in it. I use it all the time.
0 of 1 people found the following review helpful
5.0 out of 5 stars One of the best in enzymology 21 Feb 2013
By JJ - Published on Amazon.com
Format:Hardcover|Verified Purchase
Graduate students, if you are studying enzymes now and if you plan to have a career in enzymology, this is a book you ought to have.
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