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DNA Viruses: A Practical Approach (Practical Approach Series) [Paperback]

Alan J. Cann (Editor)

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Product details

Paperback: 334 pages
Publisher: OUP Oxford (21 Oct 1999)
Language English
ISBN-10: 0199637180
ISBN-13: 978-0199637188
Product Dimensions: 23.4 x 15.6 x 1.8 cm
Amazon Bestsellers Rank: 2,279,110 in Books (See Top 100 in Books)
- #80 in Books > Science & Nature > Biological Sciences > Microbiology > Virology

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Product Description

DNA Viruses: A Practical Approach groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinical isolates. Chapter 1 provides an overview of the extraction, purification and characterizations of virus DNA, but also covers the fundamentals of DNA virus culture. Chapters 2 and 3 describe approaches to the molecular investigation and mutagenesis of DNA virus genomes. Chapter 4 considers DNA virus replication and then chapters 5 & 6 describe methods to study transcription control. Chapters 7 to 9 consider aspects of the pathogenesis of DNA virus infections. The final chapter describes the current technology being applied to the development of DNA virus vectors for gene delivery. This volume will therefore be of interest to all those working on DNA viruses whether in academia, industry or clinical research.

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First Sentence
Since viruses will only replicate within living cells, special methods have to be employed for growth in vitro. Read the first page

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3 of 3 people found the following review helpful

DNA Viruses: A Practical Approach 7 April 2000

By A Customer - Published on Amazon.com

Format:Paperback

DNA Viruses: A Practical Approach describes in complete detail all of the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and real-time PCR analysis of drug resistance mutations. The book begins by describing the culture of DNA viruses and the extraction, purification and characterization of virus DNA. Other topics then covered include virus genome mapping; correlation of genotype with phenotype; sequence analysis; mutagenesis of DNA virus genomes; identification and purification of proteins involved in DNA virus replication; analysis of virus replication at the molecular level; analysis of viral gene transcription and its control; identification and study of trans-acting regulatory proteins; identification, purification and characterization of viral cytokine-binding proteins; analysis of DNA virus proteins involved in neoplastic transformation; the development of DNA virus vectors for gene delivery; chemotherapy of DNA virus infections. This volume is authoritative, comprehensive and fully up-to-date. It is an essential laboratory manual and reference for all those working on DNA viruses whether in academia, industry or clinical research.

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