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Culture of Animal Cells: A Manual of Basic Technique, 4th Edition (Wiley-Liss Publication) Hardcover – 29 Mar 2000

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Product details

  • Hardcover: 608 pages
  • Publisher: Wiley-Blackwell; 4th Edition edition (29 Mar. 2000)
  • Language: English
  • ISBN-10: 0471348899
  • ISBN-13: 978-0471348894
  • Product Dimensions: 22.3 x 3.5 x 28.9 cm
  • Average Customer Review: 4.0 out of 5 stars  See all reviews (1 customer review)
  • Amazon Bestsellers Rank: 1,770,711 in Books (See Top 100 in Books)
  • See Complete Table of Contents

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Product Description


"...Freshney provides 28 chapters that reflect the latest progress in media, specialized techniques, biotechnology, DNA transfer and tumor culture." (SciTech Book News, March 2001)

"Labs that intensively make use of cell culture, will find Culture of Animal Cells a helpful reference and great training source." ––Yale Journal of Biology and Medicine, 74, 2001.

From the Back Cover

The fourth edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made. This edition has five new chapters expanding on serum–free media, scale–up and biofermentors, molecular techniques, immortalization, and troubleshooting.

The advantages of tissue culture go beyond control of the physiochemical environment and physiological conditions as shown in the comprehensive coverage of tissue culture topics, both organ culture and cell culture, provided in this manual. A wide range of essential information from basic to specialized procedures is presented, highlighting advantages and limitations, and illustrating the properties of different types of culture.

This crucial reference for cell culture techniques includes:
∗ New Atlas of Cells section in full–color presentation
∗ Extended coverage of molecular techniques, scale–up, and serum–free medium
∗ New chapter on problem solving
∗ Photographs of cell lines, contaminations, and equipment
∗ Clear and concise tables and charts
∗ Educated recommendations on safety issues, ethical consent, and ownership
Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference.

Inside This Book

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The validity of the cultured cell as a model of physiological function in vivo has frequently been criticized. Read the first page
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Most Helpful Customer Reviews

2 of 2 people found the following review helpful By on 6 Mar. 2002
Format: Hardcover
A good overview of current techniques is provided by this text book. I would highly recommend this title to undergraduates and graduates about to embark on a cell culture project. However, as with any book covering such a wide range of material, there are some areas that are skirted over or not fully explored. The colour plates are useful but there could be more. The list of companies is very useful as sometimes it is difficult to know where to start, although I guess this section will date quickly. The problem solving section is the most useful and almost indispensible!! Overall, a good 'manual of basic technique.
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Most Helpful Customer Reviews on (beta) 11 reviews
22 of 23 people found the following review helpful
A SUPERB Manual of Basic Cell CultureTechnique 12 Sept. 2001
By P. Barriga - Published on
Format: Hardcover
I purchased this book a year ago to use in a clinical virology laboratory. Although many of the recipes used in my field are essentially set, this book allowed for broad learning in the skill of cell culture. I learned many principles of cell culture that were poorly explained or not mentioned in clinical literature and books.
This book is well laid out, the chapters well written, and the information is comprehensive. Subjects include (besides the usual stuff of cell culture) chapters covering equipment materials, safety, contamination, scale up, a reagent appendix, and supplier's listing.
This book is an excellent choice for the serious beginner. The expert will certainly find this book of value because even experts are only expert at what they do routinely and there is so much to know. This would be a great training source for research or clinical laboratories who perform cell culture.
10 of 10 people found the following review helpful
The best book on cell culture 8 Jan. 2005
By Mike_brazil - Published on
Format: Hardcover Verified Purchase
Freshney's Culture of Animal Cells is a great book. It is writen on an easy style, it is concise and complete at the same time and it does teach you all the basics (and a little bit more) of cell culture. When I was doing my masters I didn't have a lot of help with my cell culture experiments and this book was fundamental for my research. The book covers everything, starting from the biology of cultured cells, equipments, design of the lab and asseptic technique and going all the way to primary culture, cloning and selection, cell separation, characterization, differentiation and more. The book (hard cover) is pretty resistant and it easily stands going to the bench (where you surely will be using it a lot). If you are thinking about spending money on a book about cell culture techniques this is the book! Don't think twice.
1 of 1 people found the following review helpful
Best Basic Book on Tisssue Culture 7 July 2006
By Constance Oliver - Published on
Format: Hardcover
For many years Freshney's Culture of Animal Cells has been the gold standard of tissue culture reference books. This new edition adds and updates many techniques and continues to be the best book in its area. I use it extensively both for my own research and in teaching classes on tissue culture.
The bible of cell culture 10 May 2012
By Bonnie B - Published on
Format: Hardcover
I've recommended this book to so many people that I thought I'd better come write a review for it. I learned cell culture seven or eight years ago using the 4th edition, which is still excellent. The 5th edition is even better.

Cell culture is both an art and a science, and this book captures both the nitty-gritty details ("How much KCl is in DMEM?") and the aspects that are harder to quantify ("How do I learn to recognize good cell morphology?"). It is both a handy reference guide and an in-depth teaching tool.
Fantastic book! 8 Sept. 2007
By M. Levin - Published on
Format: Hardcover
Based on protocols in this book, I devised methods to complete my own experiments. I found the insights contained in the book completely indispensable. The range of topics it contains and the detail with which Freshney explains each point made the process of designing conditions for experiments rational and approachable. I highly recommend this book.
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